plasmid encoding erα (Addgene inc)

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Addgene inc plasmid encoding erα
In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and <t>ERα</t> (red dots) in MCF-7 cells and <t>transfected</t> <t>HEK293T</t> cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and <t>ERα</t> (red dots) in MCF-7 cells and <t>transfected</t> <t>HEK293T</t> cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

Plasmid Encoding Erα, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+er+alpha/pEGFP-C1-ER+alpha+(Plasmid+%2328230)/bio_rxiv__64898__2026__03__17__712321-363-9-14
Average 93 stars, based on 47 article reviews

plasmid encoding erα - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "Antidepressants interact with sex steroid receptors and their intracellular signaling components"

Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components

Journal: bioRxiv

doi: 10.64898/2026.03.17.712321

In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

Figure Legend Snippet: In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

Techniques Used: In Situ, Transfection, Expressing, Staining

Amplification:

Article Title: Paused RNA polymerase primes promoters via RNA-mediated stabilisation of transcription factor ERα.
Article Snippet: .. EFS, ERα and Halo were separately amplified from pHAGEEFS-N22p-3XRFPnls (Addgene: 75387), pEGFP-C1-ER alpha (Addgene: 28230) and pcDNA5-H2B_Halo_T2A_EGFP (Addgene:135444), respectively46–48. .. All the fragmentswere then subjected toGoldenGate assembly with BsaI-v2 (R3733L, NEB).

Article Title: Paused RNA polymerase primes promoters via RNA-mediated stabilisation of transcription factor ERα
Article Snippet: .. EFS, ERα and Halo were separately amplified from pHAGE-EFS-N22p-3XRFPnls (Addgene: 75387), pEGFP-C1-ER alpha (Addgene: 28230) and pcDNA5-H2B_Halo_T2A_EGFP (Addgene:135444), respectively – . .. All the fragments were then subjected to Golden Gate assembly with BsaI-v2 (R3733L, NEB).

Article Title: Novel Estrogen Receptor Dimerization BRET-Based Biosensors for Screening Estrogenic Endocrine-Disrupting Chemicals
Article Snippet: Nano-luciferase was amplified from pcDNA3.1-CAG-NanoLuc-VChR1-EYFP (Addgene #114112) and digested with NotI/XbaI. .. ERα and ERβ were amplified from pEGFP-C1-ER alpha (Addgene #28230) and pEGFP-C1-ER beta (Addgene #28237), respectively. ..

Article Title: Paused Polymerase synergises with transcription factors and RNA to enhance the transcriptional potential of promoters
Article Snippet: .. EFS, ERα and Halo were separately amplified from pHAGE-EFS-N22p-3XRFPnls (Addgene: 75387), pEGFP-C1-ER alpha (Addgene: 28230) and pcDNA5-H2B_Halo_T2A_EGFP (Addgene:135444) respectively ( ; ; ). .. All the fragments were then subjected to golden gate assembly with BsaI-v2 (R3733L, NEB).

Multiple Displacement Amplification:

Article Title: Estrogen receptor alpha dynamics and plasticity during endocrine resistance.
Article Snippet: All the images were analyzed using Nikon’s NIS element software. .. The MDA-MB-231 cells were used to transfect the pEGFP-C1-ER alpha, a gift from Michael Mancini (Addgene plasmid # 28230) [27]. .. Following the manufacturer’s instructions, the plasmid was transfected using a Neon Electroporation system (Invitrogen, USA).

Article Title: Estrogen receptor alpha dynamics and plasticity during endocrine resistance
Article Snippet: All the images were analyzed using Nikon's NIS element software. .. The MDA-MB-231 cells were used to transfect the pEGFP-C1-ER alpha, a gift from Michael Mancini (Addgene plasmid # 28230) [ ]. .. Following the manufacturer's instructions, the plasmid was transfected using a Neon Electroporation system (Invitrogen, USA).

Plasmid Preparation:

Article Title: A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.
Article Snippet: 100 Restriction enzymes BamHI, HindIII, NdeI and NcoI were obtained from Thermo Fisher 101 Scientific. .. Bacterial strains DH5α and BL21(DE3) were obtained from the National 102 BioResource Project Escherichia coli strain. pEGFP-C1-ER alpha was a gift from Michael 103 Mancini (Addgene plasmid # 28230; http://n2t.net/addgene:28230; RRID: Addgene_28230). .. 104 pmTurquoise2-ER was a gift from Dorus Gadella (Addgene plasmid # 36204; 105 http://n2t.net/addgene:36204; RRID: Addgene_36204).

Article Title: Loss of estrogen receptor alpha promotes distant metastasis through epithelial-mesenchymal transition in luminal-type breast cancer.
Article Snippet: .. The pEGFP-C1-ER alpha (Plasmid #28230) were purchased from Addgene. ..

Review